Anti-candida activities of four bacterial endophytic extracts isolated from Urena lobata.

Volume 8, Issue 2, April 2023     |     PP. 61-77      |     PDF (709 K)    |     Pub. Date: April 9, 2023
DOI: 10.54647/biology180278    82 Downloads     195347 Views  

Author(s)

Jean Baptiste Hzounda Fokou, Microbial Product Research Unit, Laboratory of Pharmacology, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon
Giselle Marguerite Etame Loe, Laboratory of galenic, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon.
Clive Jezeh Ngueguim, Microbial Product Research Unit, Laboratory of Pharmacology, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon
Francine Kouemo, Microbiology service, Gynecoobstetric and Pediatric Hospital of Douala
Abi Edmund Otang, Microbial Product Research Unit, Laboratory of Pharmacology, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon
Guy Pascal Ngaba, Laboratory of Microbiology, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon
Jules Clement Assob, Laboratory of Microbiology, Faculty of Medicine and Pharmaceutical Sciences, PO Box 2701 Douala, Cameroon

Abstract
Purpose: Most antifungals have drawbacks, including toxicity, efficacy, and cost, and their misuse has led to the emergence of resistant strains. Therefore, more is needed. This study aimed to evaluate the antifungal properties of bacterial endophytic extracts on Candida spp.
Materials and Methods: Biological samples were bacterial endophytes from Urena lobata and Candida species isolated from Stool. The Candida species were purified on chromogenic aga medium. The MIC was determined using broth microdilution starting from 200 µg/ml as the highest concentration. The bacterial endophytes were characterised based on morphology and motility. The inhibitory parameters, time kill kinetics assay and probable site of action of the extract were determined using the cell counting method and microscopic observation under methylene blue stain.
Results:The characterised Candida species were Candida albicans, Candida glabrata, Candida dubliniensis, Candida parapsilosis, Candida tropicalis and Candida krusei. The selected extracts from screening were A23, A34, A52, A64, A80, and A96. The bacteria producing these extracts were all atypical nonmotile and were Streptobacillus spp. and Bacillus spp. The MIC ranged between 100 µg/ml and 200 µg/ml. The time kill kinetics assay revealed that the cidal effect of the extracts started after 3 to 9 hours of contact with the extracts. The shape of the cells posttreatment revealed that the probable site of action is on the cell membrane for A23 and the cell wall, nucleus, or mitochondria for A52, A64 and A80.
Conclusion: Bacterial endophytes have been proven through this study to be a suitable lead compound in antifungal resistance drug development.

Keywords
Candida spp, Antifungal, Bacterial Endophytes, Urena lobata, Gut

Cite this paper
Jean Baptiste Hzounda Fokou, Giselle Marguerite Etame Loe, Clive Jezeh Ngueguim, Francine Kouemo, Abi Edmund Otang, Guy Pascal Ngaba, Jules Clement Assob, Anti-candida activities of four bacterial endophytic extracts isolated from Urena lobata. , SCIREA Journal of Biology. Volume 8, Issue 2, April 2023 | PP. 61-77. 10.54647/biology180278

References

[ 1 ] Pfaller MA. Epidemiology of candidiasis. J Hosp Infect. 1995;30:329–38.
[ 2 ] Ruhnke M. Epidemiology of Candida albicans Infections and Role of Non-Candida-albicans Yeasts. Vol. 7, Current Drug Targets. 2006.
[ 3 ] Bennett JE (John E, Dolin R, Blaser MJ. Mandell, Douglas, and Bennett’s principles and practice of infectious diseases. 9th ed. Bennett JE, Dolin R, Blaser MJ, editors. philadelphia: Elsevier; 2020. 4895 p.
[ 4 ] Banki K, Bluth MH, Bock JL, Bowne WB, Hutchison RE, Karcher DS, et al. HENRY’S CLINICAL DIAGNOSIS AND MANAGEMENT BY LABORATORY METHODS. 24th ed. McPherson RA, Pincus MR, editors. philadelphia: elsvier; 2022. 1956 p.
[ 5 ] Lehman LG, Kangam L, Mbenoun ML, Nguepi EZ, Essomba N, Tonga C, et al. Intestinal parasitic and candida infection associated with HIV infection in Cameroon. J Infect Dev Ctries. 2013;7(2):137–43.
[ 6 ] Kontoyiannis DP. Antifungal resistance: An emerging reality and a global challenge. J Infect Dis. 2017 Jan 15;216:S431–5.
[ 7 ] Ngouana TK, Toghueo RMK, Kenfack IF, Lachaud L, Nana AK, Tadjou L, et al. Epidemiology and antifungal susceptibility testing of non-albicans Candida species colonizing mucosae of HIV-infected patients in Yaoundé (Cameroon). J Mycol Med. 2019 Sep 1;29(3):233–8.
[ 8 ] Sardi JCO, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Giannini MJS. Candida species: Current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol. 2013;62(PART1):10–24.
[ 9 ] Kumar A, Radhakrishnan E. K. Microbial endophytes : functional biology and applications. Kumar A, Radhakrishnan E. K, editors. Charlotte Cockle; 2020. 404 p.
[ 10 ] Hong CE, Jo SH, Jo IH, Park JM. Diversity and antifungal activity of endophytic bacteria associated with Panax ginseng seedlings. Plant Biotechnol Rep. 2018 Dec 1;12(6):409–18.
[ 11 ] Gao X, Gong Y, Huo Y, Han Q, Kang Z, Huang L. Endophytic bacillus subtilis strain E1R-J is a promising biocontrol agent for wheat powdery mildew. Biomed Res Int. 2015;1–8.
[ 12 ] Strobel GA. Endophytes as sources of bioactive products. microbes Infect. 2003;5:535–44.
[ 13 ] Vasundhara M, Reddy MS, Kumar A. Secondary Metabolites From Endophytic Fungi and Their Biological Activities [Internet]. New and Future Developments in Microbial Biotechnology and Bioengineering. Elsevier B.V.; 2019. 237–258 p. Available from: http://dx.doi.org/10.1016/B978-0-444-63504-4.00018-9
[ 14 ] Kusari S, Singh S, Jayabaskaran C. Biotechnological potential of plant-associated endophytic fungi : hope versus hype. Trends Biotechnol [Internet]. 2014;32(6):297–303. Available from: http://dx.doi.org/10.1016/j.tibtech.2014.03.009
[ 15 ] Gond SK, Mishra A, Sharma VK, Verma SK, Kumar J, Kharwar RN, et al. Diversity and antimicrobial activity of endophytic fungi isolated from Nyctanthes arbor-tristis, a well-known medicinal plant of India. Mycoscience. 2012;53(2):113–21.
[ 16 ] Christina A, Christapher V, Bhore S. Endophytic bacteria as a source of novel antibiotics: An overview. Vol. 7, Pharmacognosy Reviews. 2013. p. 11–6.
[ 17 ] Singh M, Kumar A, Singh R, Pandey KD. Endophytic bacteria: a new source of bioactive compounds. Vol. 7, 3 Biotech. Springer Verlag; 2017. p. 315–29.
[ 18 ] Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN. Bacterial endophytes: Recent developments and applications. Vol. 278, FEMS Microbiology Letters. 2008. p. 1–9.
[ 19 ] Hzounda Fokou J, Pk L, Er M. Antibacterial and Antioxidant Properties of Endophytic Fungi Extracts from Cola acuminata ( Sterculiaceae ). Austin J Pharmacol Ther. 2022;10(1):1159–67.
[ 20 ] Rani R, Sharma D, Chaturvedi M, Parkash Yadav J. Antibacterial Activity of Twenty Different Endophytic Fungi Isolated from Calotropis procera and Time Kill Assay. Clin Microbiol Open Access. 2017;06(03).
[ 21 ] Xie S, Liu J, Gu S, Chen X, Jiang H, Ding T. Antifungal activity of volatile compounds produced by endophytic Bacillus subtilis DZSY21 against Curvularia lunata. Ann Microbiol. 2020 Feb 24;70(2):1–10.
[ 22 ] Heroine MM, Jean Baptiste HF, Victorine Lorette Y, Rufin Marie TK, Pythagore FS, Pierre E, et al. Isolation of endophytics fungi from Cola acuminata Schott & Endl, and antifungal activity against Candida Sp. Eur J Biol Biotechnol. 2020 Sep 5;1(5).
[ 23 ] Omonkhua AA, Onoagbe IO. Evaluation of the long-term effects of Urena lobata root extracts on blood glucose and hepatic function of normal rabbits. J Toxicol Environ Heal Sci [Internet]. 2011;3(8):204–13. Available from: http://www.academicjournals.org/JTEHS
[ 24 ] Kumar   Dinesh, Kumar S, Sahu M, Kumar A. Evaluation of Pharmacological Features and Nanoparticle Formation by Urena lobata. Haya Saudi J Life Sci. 2020;5(10):226–35.
[ 25 ] Fagbohun ED, Asare RR, Egbebi AO. Chemical composition and antimicrobial activities of Urena lobata L. (Malvaceae). J Med Plants Res. 2014;6(12):22562260.
[ 26 ] Taufiq MMJ, Darah I. Anti-candidal activity of ethyl acetate crude extract of endophytic fungus, lasiodiplodia pseudotheobromae ibrl os-64 against candida albicans. Int J Pharm Res. 2020;12(September):257–65.
[ 27 ] Begum K, Mannan SJ, Rezwan R, Rahman S, Nur-e-kamal A. Isolation and Characterization of Bacteria with Biochemical and Pharmacological Importance from Soil Samples of Dhaka City. 2017;
[ 28 ] Principle M, Procedure T, Results I. Chromatic Candida. 2015;
[ 29 ] Tenover FC. Antibiotic Susceptibility Testing. Encycl Microbiol. 2009 Jan;67–77.
[ 30 ] Sen A, Batra A. Determination of antimicrobial potentialities of different solvent extracts of the medicinal plant: Phyllanthus amarus Schum. and Thonn. Int J Green Pharm. 2012;6(1):50–6.
[ 31 ] Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71–9.
[ 32 ] Leite MCA, De Brito Bezerra AP, De Sousa JP, Guerra FQS, De Oliveira Lima E. Evaluation of antifungal activity and mechanism of action of citral against Candida albicans. Evidence-based Complement Altern Med. 2014;2014.
[ 33 ] Adusei EBA, Adosraku RK, Oppong-kyekyeku J, Amengor CDK, Jibira Y. Resistance Modulation Action , Time-Kill Kinetics Assay , and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn. 2019;2019.
[ 34 ] Tan D, Fu L, Han B, Sun X, Zheng P, Zhang J. Identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana Fusarium wilt. PLoS One. 2015 Jul 2;10(7):1–14.
[ 35 ] Rat A, Naranjo HD, Krigas N, Grigoriadou K, Maloupa E, Alonso AV, et al. Endophytic Bacteria From the Roots of the Medicinal Plant Alkanna tinctoria Tausch (Boraginaceae): Exploration of Plant Growth Promoting Properties and Potential Role in the Production of Plant Secondary Metabolites. Front Microbiol. 2021;12(February):1–14.
[ 36 ] Al-Nadabi HH, Al-Buraiki NS, Al-Nabhani AA, Maharachchikumbura SN, Velazhahan R, Al-Sadi AM. In vitro antifungal activity of endophytic bacteria isolated from date palm (Phoenix doctylifera L.) against fungal pathogens causing leaf spot of date palm. Egypt J Biol Pest Control. 2021 Dec;31(1):1–8.
[ 37 ] Devi R, Nath T, Boruah RR, Darphang B, Nath PK. Antimicrobial activity of bacterial endophytes from Chirata ( Swertia chirata Wall .) and Datura ( Datura stramonium L .). Egypt J Biol Pest Control. 2021;31(69):1–7.
[ 38 ] Das G, Park S, Choi J, Baek KH. Anticandidal potential of endophytic bacteria isolated from Dryopteris uniformis (makino). Jundishapur J Microbiol. 2019;12(1):1–10.
[ 39 ] Whaley SG, Berkow EL, Rybak JM, Nishimoto AT, Barker KS, Rogers PD. Azole antifungal resistance in Candida albicans and emerging non-albicans Candida Species. Vol. 7, Frontiers in Microbiology. Frontiers Media S.A.; 2017. p. 2173–85.
[ 40 ] Singh G, Urhekar A. CANDIDAL INFECTION: EPIDEMIOLOGY, PATHOGENESIS AND RECENT ADVANCES FOR DIAGNOSIS Virulence Factors Detection in Aspergillus Isolates from Clinical and Environmental Samples View project CANDIDAL INFECTION: EPIDEMIOLOGY, PATHOGENESIS AND RECENT ADVANCES FO. Bull Pharm Med Sci [Internet]. 2013;1(1):1–8. Available from: http://www.bopams.com