Decoupling of cell refractive index and thickness distribution under three-wavelength phase imaging

Volume 6, Issue 5, October 2021     |     PP. 293-308      |     PDF (643 K)    |     Pub. Date: August 15, 2021
DOI: 10.54647/cm32587    78 Downloads     4627 Views  

Author(s)

Jingrong Liao, School of Mechanical Engineering, Jiangsu University, Zhenjiang 212013, China
Yawei Wang, School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, China
Shuangshuang Xue, School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, China
Yujuan Sun, School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, China
Wen Jiang, School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, China
Yuanyuan Xu, School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, China

Abstract
Decoupling of refractive index (RI) and thickness information of a cell is very importment in cell’s analysis. This paper proposes a method for decoupling the refractive index and thickness of a cell under three-wavelength phase imaging. Based on the Quantitative Phase Imaging (QPI) method, the proposed method can obtain the sample's refractive index and thickness distributions by solving three-wavelength phase equations using the approximate setting under dispersion based on the optical and optical dispersion theories. In simulation experiments, the obtained refractive index and thickness distribution results have high accuracy with a maximum error lower than 6%. The validation results show that the method can effectively decouple the refractive index and thickness for homogeneous cells and multi-media non-spherical cells. That demonstrates the method's effectiveness, making up for the shortcomings of traditional methods and playing an important role in 3D imaging of the cell’s sub-structures.

Keywords
Quantitative phase imaging; cellular substructure; three-wavelength; decoupling refractive index; thickness distribution.

Cite this paper
Jingrong Liao, Yawei Wang, Shuangshuang Xue, Yujuan Sun, Wen Jiang, Yuanyuan Xu, Decoupling of cell refractive index and thickness distribution under three-wavelength phase imaging , SCIREA Journal of Clinical Medicine. Volume 6, Issue 5, October 2021 | PP. 293-308. 10.54647/cm32587

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