Jinpeng Liu, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan,Shandong 250014, China

Ranran Cheng, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan,Shandong 250014, China

Zhongni Wang, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, PR China

Jinying Wang, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan,Shandong 250014, China

Jun Liu, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China

Feng Liu, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan,Shandong 250014, China

Abstract
Wood vinegars without secondary pollutants are extracted from plant material．Recently, research on wood vinegars has received increasing attention because of the rising awareness of environmental protection. Herein, we first exploited the wood vinegar obtained from peony which has never been discovered. This wood vinegar liquor was extracted by dry distillation and collected with four different methods which included different temperature sections of 0 ~ 110 C, 110 ~ 150 C and different attached containers of condenser pipe, flask (M1, M2, M3 and M4 respectively). The chemical compositions of every segment were analyzed by gas chromatography-mass spectrometry (GC-MS). In addition, the antioxidant activities of DPPH free radical and superoxide anion detection were investigated. We also developed the anti-inflammatory activity of every dry distillation with RAW 264.7 cell line. The result showed that dry distillations collected at two different temperature have a great antioxidant activity compared with vitamin C. Meanwhile, it revealed a powerful anti-inflammatory on these two dry distillations. This research provided a novel research direction for high-value utilization of waste plants on peony, which can be used as food additives or drugs in the future.

Keywords
Peony; Wood vinegar; Dry distillation; Antioxidant activity; Anti-inflammatory activity

Cite this paper
Jinpeng Liu, Ranran Cheng, Zhongni Wang, Jinying Wang, Jun Liu, Feng Liu, Study on Preparation and Bioactivity of Peony Wood Vinegar , SCIREA Journal of Agriculture. Volume 5, Issue 2, April 2020 | PP. 16-28.

References

[ 1 ]	SY, YQ, Gao and JS, "The Effect of Wood Vinegar on Hydrothermal Liquefaction of Cotton Stalk Under CO Atmosphere," ENERG SOURCE PART A, 36 (4), 411-417. 2014.
[ 2 ]	Shiny, K. S. and Remadevi, O. K., "Evaluation of termiticidal activity of coconut shell oil and its comparison to commercial wood preservatives," European Journal of Wood & Wood Products, 72, 139–141. 2014.
[ 3 ]	Niamsa, B. N., "Study on wood vinegars for use as coagulating and antifungal agents on the production of natural rubber sheets," Biomass and Bioenergy, 33 (6) 994-998, 2009.
[ 4 ]	Oramahi, H. A. and Yoshimura, T., "Antifungal and antitermitic activities of wood vinegar from Vitex pubescens Vahl," Journal of Wood Science, 59 (4), 344-350. 2013.
[ 5 ]	Akakabe, Y., Tamura, Y., Iwamoto, S., Takabayashi, M. and Nyuugaku, T., "Volatile Organic Compounds with Characteristic Odor in Bamboo Vinegar," Journal of the Agricultural Chemical Society of Japan, 70 (11), 2797-2799. 2006.
[ 6 ]	Cai, K., Jiang, S., Ren, C. and He, Y., "Significant damage-rescuing effects of wood vinegar extract in living Caenorhabditis elegans under oxidative stress," Journal of the ence of Food & Agriculture, 92 (1), 29-36. 2011.
[ 7 ]	Mun, S. P. and Ku, C. S., "Pyrolysis GC-MS analysis of tars formed during the aging of wood and bamboo crude vinegars," Journal of Wood ence, 56 (1), 47-52. 2010.
[ 8 ]	Zhao, N., Xin, H., Li, Z., Wang, Z. and Zhang, L., "Supplemental Feeding of Laying Hens with Wood Vinegar to Decrease the Ratio of n-6 to n-3 Fatty Acids in Eggs," Chemical Research in Chinese Universities, 35 (6), 983-989. 2019.
[ 9 ]	Yatagai, M., Nishimoto, M., Hori, K., Ohira, T. and Shibata, A., "Termiticidal activity of wood vinegar, its components and their homologues," Journal of Wood ence, 48 (4), 338-342. 2002.
[ 10 ]	Ma, C., Song, K., Yu, J., Yang, L., Zhao, C., Wang, W., Zu, G. and Zu, Y., "Pyrolysis process and antioxidant activity of pyroligneous acid from Rosmarinus officinalis leaves," Journal of Analytical and Applied Pyrolysis, 104, 38-47. 2013.
[ 11 ]	Velmurugan, N., Han, S. S. and Lee, Y. S., "Antifungal Activity of Neutralized Wood Vinegar with Water Extracts of Pinus densiflora and Quercus serrata Saw Dusts," International Journal of Environmental Research, 3 (2), 167-176. 2009.
[ 12 ]	Loo, A. Y., Jain, K. and Darah, I., "Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata," Food Chemistry, 107 (3), 1151-1160. 2008.
[ 13 ]	Mu, J., Uehara, T. and Furuno, T., "Effect of bamboo vinegar on regulation of germination and radicle growth of seed plants," Journal of Wood ence, 49 (3), 262-270. 2003.
[ 14 ]	Qiaomei, Wu, Shouyu, Zhang, Baoxin, Hou, Hongjun, Zheng, Wenxiang and Deng, "Study on the preparation of wood vinegar from biomass residues by carbonization process," Bioresource Technology, 179, 98-103. 2015.
[ 15 ]	Li, J., Wang, C. and Yang, Z., "Production and separation of phenols from biomass-derived bio-petroleum," Journal of Analytical & Applied Pyrolysis, 89 (2), 218-224. 2010.
[ 16 ]	Loo, A. Y., Jain, K. and Darah, I., "Antioxidant and radical scavenging activities of the pyroligneous acid from a mangrove plant, Rhizophora apiculata," Food Chemistry, 104 (1), 300-307. 2007.
[ 17 ]	Sun, H., Feng, Y., Xue, L., Mandal, S. and Yang, L., "Responses of ammonia volatilization from rice paddy soil to application of wood vinegar alone or combined with biochar," Chemosphere, 242, 125247. 2020.
[ 18 ]	Luo, X., Wang, Z., Meki, K., Wang, X. and Li, F., "Effect of co-application of wood vinegar and biochar on seed germination and seedling growth," Journal of soil & sediments, 19 (12), 3934-3944. 2019.
[ 19 ]	Dos Santos, N., Tose, L., Da Silva, S., Murgu, M., Kuster, R., Ortiz, R., Camargo, F., Vaz, B., Lacerda, V. and Romão, W., "Analysis of isomeric cannabinoid standards and cannabis products by UPLCESITWIMMS: A comparison with GCMS and GC × GCQMS," Journal of the Brazilian Chemical Society, 30, 60-70. 2019
[ 20 ]	Roy, C., Maji, S. and Ghose, S., "Stress causing dynamic changes of four phytohormones in tobacco and tomato: A GC-MS analysis," 9, 16-22. 2019.
[ 21 ]	Sassaki, G. L., Gorin, P. A. J., Souza, L. M., Czelusniak, P. A. and Iacomini, M., "Rapid synthesis of partially O-methylated alditol acetate standards for GC-MS: some relative activities of hydroxyl groups of methyl glycopyranosides on Purdie methylation," Carbohydrate Research, 340 (4), 731-739. 2005.
[ 22 ]	Sridhar, K. and Charles, A. L., "In vitro antioxidant activity of Kyoho grape extracts in DPPH' and ABTS assays: Estimation methods for EC_(50) using advanced statistical programs," Food Chemistry, 275, 41-49. 2019.
[ 23 ]	Chedea, V. S., "Total Polyphenols Content and Antioxidant DPPH Assays on Biological Samples," Polyphenols in Plants (Second Edition), 169-183. 2019.
[ 24 ]	Petchpoung, K., Soiklom, S., Siri-anusornsak, W., Khlangsap, N., Tara, A. and Maneeboon, T., "Predicting antioxidant activity of wood vinegar using color and spectrophotometric parameters," MethodsX, 7, 100783. 2020.
[ 25 ]	Ngamwongsatit, P., Banada, P. P., Panbangred, W. and Bhunia, A. K., "WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line," J Microbiol Methods, 73 (3), 211-215. 2008.
[ 26 ]	Wang, Q. S., Cui, Y. L., Wang, Y. F. and Chi, W., "Effects of compounds from Bi-Qi Capsule on the expression of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 macrophages," Journal of Ethnopharmacology, 136 (3), 480-487. 2011.