An effective method for high purity genomic DNA extraction from N. flagelliforme

Volume 1, Issue 2, December 2016     |     PP. 169-185      |     PDF (576 K)    |     Pub. Date: December 26, 2016
DOI:    396 Downloads     7431 Views  

Author(s)

Wen-Jin Ma, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China; The Institute of Gansu Province Light Industrial Scientific Research, Lanzhou 730000, China
Xue-Feng Chen, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China
Wen-Jin Lou, Gansu Provincial Academic Industrial for Medical Research, Lanzhou 620100, China
Huan Liu, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China
Bo Wang, The Institute of Gansu Province Light Industrial Scientific Research, Lanzhou 730000, China
Qian Wu, Gansu Provincial Academic Industrial for Medical Research, Lanzhou 620100, China
Yu Zhao, The Institute of Gansu Province Light Industrial Scientific Research, Lanzhou 730000, China
Tao Peng, The Institute of Gansu Province Light Industrial Scientific Research, Lanzhou 730000, China
Peng Chen, School of Pharmacy, Lanzhou University, Donggang West Road No. 199, Lanzhou, 730020, PR China

Abstract
An effective method for high purity genomic DNA extraction from N. flagelliforme, which was four steps including 1) the obtaining of pure cultured cells of N. flagelliforme by liquid culture, 2) the cleaning of cells by the cleaning solution, 3) disruption of cell walls by the glass homogenizer, and 4) precipitation of polysaccharide and protein and other interfering substance. The comparison with bacterial DNA extraction kit clearly indicates that the quality of DNA with the OD260/OD280 ratio of 1.8, and the gel electrophoresis analysis revealed high quality and high yield of genomic DNA extracted by this method. Furthermore, the new method is also useful for other cyanobacterial DNA isolation. The method does not require lysozyme, phenol extraction, and the genomic DNA of N. flagelliforme thus extracted by this method is of high quantity as well as quality and can further be used directly for PCR amplification and genetic recombination.

Keywords
N. flagelliforme; Genomic DNA extraction; Effective method; Agarose gel electrophoresis; PCR verification

Cite this paper
Wen-Jin Ma, Xue-Feng Chen, Wen-Jin Lou, Huan Liu, Bo Wang, Qian Wu, Yu Zhao, Tao Peng, Peng Chen, An effective method for high purity genomic DNA extraction from N. flagelliforme , SCIREA Journal of Agriculture. Volume 1, Issue 2, December 2016 | PP. 169-185.

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